Tuesday 31 October 2023

Problems in Crop improvement in INDIA (Author: Prashant B. Kale (Ph.D.))

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Crop improvement involves various categories of problems

Author: Prashant B. Kale (Ph.D.)

1. Genetic and Breeding Problems:

1.1. Genetic Diversity: Limited genetic diversity can hinder the development of new crop varieties, making crops vulnerable to pests, diseases, and environmental changes.

1.2. Inbreeding Depression: Repeated use of closely related individuals in breeding programs can lead to a decrease in vigor and overall plant health.

1.3. Heterosis or Hybrid Vigor: Achieving and maintaining hybrid vigor can be challenging, as it often requires crossing two genetically diverse parents.

1.4. Genetic Barriers: Crossbreeding between species or genera can be technically challenging, limiting the expansion of genetic diversity.

Notes: Crop Improvement in India (Author: Prashant B. Kale (Ph.D.))

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Status of Crop Improvement in India

Author: Prashant B. Kale (Ph.D.)

Crop improvement in India has a long-standing history dating back to ancient agricultural practices. Over the years, this essential sector has evolved significantly, adopting modern techniques and technologies to meet the growing demand for food, fiber, and industrial raw materials. This comprehensive report provides an overview of the present status of crop improvement in India, emphasizing the importance of this sector, the key stakeholders involved, the current challenges, and the innovative approaches being used to enhance crop yields, nutritional value, and resistance to biotic and abiotic stresses.

1.     Introduction Crop improvement in India is a crucial component of the country's agricultural sector, which plays a vital role in the overall economy and the livelihoods of millions. This report explores the various aspects of crop improvement in India, focusing on the current status, strategies, challenges, and the role of different stakeholders.

Monday 30 October 2023

"Microtomy and Microscopy in Plant Histology: Techniques and Applications" (Author: Prashant B. Kale (Ph.D.))

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 "Microtomy and Microscopy in Plant Histology: Techniques and Applications"

Author: Prashant B. Kale (Ph.D.)

A microtome is a specialized precision cutting instrument, which accurately and repeatedly slices sections from a block of embedded tissue. Different kinds of microtomes are used to section paraffin and plastic-embedded tissues as well as the specialized microtomes used to section frozen tissues.

In the case of plants, microtomy is used to prepare thin sections of plant tissues for analysis, particularly for microscopic examination of cells and sub-cellular events. Basic steps for creating microtome sections for plant tissue analysis are explained as: (figure: microtome and cryotome instruments with important parts)

Materials and Equipment:

  1. Plant specimens
  2. Microtome (a specialized instrument for cutting thin sections)
  3. Embedding medium (e.g., paraffin wax or resin)
  4. Razor blades or microtome blades
  5. Glass slides
  6. Microscope
  7. Staining solutions (optional)

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