IR data: Evaluation of Rice Genotypes and Mutants for Drought Tolerance

IR Data: Evaluation of Rice Genotypes and Mutants for Drought Tolerance Using Infrared (IR) Imaging

Principle of the Method

Infrared (IR) imaging evaluates plant temperature and water status by detecting thermal radiation emitted from plant surfaces. Plants under drought stress often exhibit increased canopy temperatures due to reduced transpiration caused by stomatal closure. By analyzing IR images, differences in canopy temperature, water-use efficiency, and drought stress responses among rice genotypes, varieties, and mutants can be quantified.

Methodology

1. Setup of IR Imaging

   • Use a thermal infrared camera to capture canopy temperature data during the vegetative and reproductive stages.
   • Conduct imaging under clear skies during midday (10:00 AM to 2:00 PM) to minimize environmental variability.

2. Data Collection

   • Capture IR images for all genotypes and mutants in well-watered (WW) and drought-stressed (DS) conditions.
   • Record concurrent environmental parameters, such as air temperature, relative humidity, and solar radiation, for normalization.

3. Image Analysis

   • Process IR images using thermal imaging software to extract average canopy temperature for each plot.
   • Normalize temperature readings based on environmental conditions and calculate the crop water stress index (CWSI) for each genotype.

4. Supplementary Measurements

   • Measure stomatal conductance and relative water content (RWC) to correlate physiological responses with IR data.
   • Validate thermal data with leaf temperature measured using a portable infrared thermometer.

Expected Output

1. Canopy Temperature Analysis

   • Identification of genotypes with lower canopy temperatures under drought stress, indicating better cooling through transpiration.
   • Categorization of mutants into tolerant and sensitive groups based on thermal profiles.

2. Crop Water Stress Index (CWSI)

   • Calculation of CWSI for each genotype/mutant to quantify the degree of drought stress experienced.
   • Genotypes with low CWSI values will be identified as drought-tolerant.

3. Trait Association

   • Correlation between canopy temperature and yield, water-use efficiency, and physiological traits (e.g., RWC and stomatal conductance).
   • Identification of traits contributing to drought resilience through thermal data.

4. Ranking of Genotypes and Mutants

   • A ranked list of genotypes and mutants based on their ability to maintain lower canopy temperatures and higher drought tolerance indices.
   • Insights into genetic and phenotypic variability in drought response.

5. Visualization

   • Heat maps and temperature distribution charts for a clear graphical representation of drought stress impacts across genotypes.

Applications of the Output

• Guide breeding programs to select drought-tolerant genotypes and mutants.
• Enhance understanding of physiological mechanisms underlying drought resilience.
• Develop predictive models for field-scale drought monitoring and management.

Title & Method: Evaluation of Rice Genotypes and Mutants for Drought Tolerance

Evaluation of Rice Genotypes and Mutants for Drought Tolerance

_Dr. PB Kale (Date of draft for proposal; April 1, 2024)

SN	Expt	Em(IM)blem	MapLocat(ions)	Address(s)	Title / Material and Method	Field Data	Cataloging	InfoBase Status
1	Expt 1: Evaluation of Rice Genotypes and Mutants for Drought Tolerance		https://maps.app.goo.gl/ZSP8e7y6iuHNrnRc8	VNGCAB-KVK Field area, Yavatmal Key Words: Rice, Drought, Rice	https://krishiprabha.blogspot.com/2025/01/evaluation-of-rice-genotypes-and.html	IR data	NO	YES
						Spectral signatures
						Phenomics data
						Genomics Data
						General articles

1. Selection of Plant Material

• Identify and procure a diverse set of rice genotypes and mutants with varying genetic backgrounds.
• Include drought-tolerant controls and sensitive checks for comparison.

2. Experimental Site Preparation

• Select well-defined experimental plots at the VNGCAB campus with controlled irrigation systems.
• Divide plots into two treatments:
• Well-Watered Condition (WW): Regular irrigation to maintain field capacity.
• Drought-Stressed Condition (DS): Water supply withheld at critical growth stages.

3. Experimental Design

• Use a Randomized Complete Block Design (RCBD) with three replicates for each genotype.
• Plot size: 5 rows per genotype, 3 meters long with 20 cm row spacing.

4. Sowing

• Date of sowing: June 15, 2024 (adjusted for monsoon onset in the region).
• Sow seeds manually, ensuring uniform spacing.

Climate Change: Understanding, Effects, and Individual Efforts for a Better Climate Condition [By Ms. Tanaya Rajeev Gajbhiye, (B.Tech. Biotechnology)]

 

Climate Change: Understanding, Effects, and Individual Efforts for a Better Climate Condition

Ms. Tanaya Rajeev Gajbhiye, (B.Tech. Biotechnology)

 The temperature in India has been skyrocketing for a decade now. Growing up during the early 2000s, I thoroughly enjoyed the summer season. The warm weather, ice- creams and staying up late are what made me really look forward for the summer vacations. However, the lovely happy summer has now turned into something unbearable since the last few years due to the tremendous rise in the temperature.

India has been experiencing constant heat waves and the temperature in places like Delhi has gone as high as 52.1°C. Everybody needs to ask themselves, why is this happening? who is responsible for these immense rises in temperature levels?  What are the repercussions if this keeps going on? and the most important question what can” I “do to help the situation? The reason behind this all is “climate change”.

What is Climate change?

First and foremost, we need to understand what climate change actually means; According to the United Nations, Climate change refers to long-term shifts in temperatures and weather patterns.

Such shifts can be natural, due to changes in the sun’s activity or large volcanic eruptions. But since the 1800s, human activities have been the main driver of climate change, primarily due to the burning of fossil fuels like coal, oil and gas.

 

RNA-Based Vaccine: Sprayable Antiviral Suspension for Plants (By Saurabh and Prashant)

  

RNA-Based Vaccine: Sprayable Antiviral Suspension for Plants

(Saurabh and Prashant)

  

Table of Contents:

·         Introduction

o   Overview of Plant Viral Diseases

o   Importance of Developing Effective Antiviral Measures for Plants

·         RNA-Based Vaccines: A New Frontier

o   Concept and Mechanism of RNA-Based Vaccines

o   Comparison with Traditional Plant Protection Methods

·         Mechanism of Action

o   How RNA-Based Vaccines Work in Plants

o   Interaction with Plant Immune System

o   Benefits over Other Antiviral Methods

·         Advantages and Benefits of dsRNA/RNAi

·         Potential Challenges and Limitations of dsRNA/RNAi RNA Vaccines

EcoCampusHub: Discover the Green Lungs of VNGCAB, YTL Campus (by PB Kale)

EcoCampusHub: Discover the Green Lungs of Our Campus (by Dr. PB Kale)

At EcoCampusHub, we're on a mission to celebrate and conserve the rich diversity of plant life that thrives right here in our college space. Our interactive database offers a window into the botanical wonders of our campus, inviting you to explore, learn, and connect with the natural world around you.

What We Offer:

1. Comprehensive Flora Database: Dive into our extensive collection of plant species found on campus, complete with detailed descriptions, photos, and ecological insights.
2. Educational Resources: Whether you're a student, faculty member, or nature enthusiast, our educational materials provide fascinating information about local flora, from common species to rare gems.
3. Citizen Science Opportunities: Join us in documenting and monitoring the plants on campus. Your observations contribute to ongoing research and conservation efforts.
4. Community Engagement: Connect with like-minded individuals passionate about plants and environmental stewardship through workshops, events, and volunteer opportunities.

Why EcoCampusHub Matters:

Our campus is not just a place of learning—it's a living ecosystem teeming with biodiversity. By exploring and understanding the flora surrounding us, we gain a deeper appreciation for the interconnectedness of all life and our role in preserving it for future generations.

Get Involved Today:

Ready to embark on a botanical adventure? Start exploring EcoCampusHub now and join us in celebrating the natural beauty of our campus.

Help Us Grow:

ü  Donate: Your support helps us expand our database, develop educational programs, and enhance campus biodiversity initiatives. Every contribution makes a difference.

ü  Contribute: Are you a botanist, ecologist, or passionate about plants? Share your expertise by contributing to our database or volunteering with our team.

Image and Information Section:

ü  Plant of the Month: Each month, we feature a standout plant species found on campus, accompanied by stunning photos and fascinating facts.

ü  Spotlight on Campus Gardens: Learn about the various gardens and green spaces on campus, their design, and the plants they showcase.

ü  Local Wildlife: Discover the interconnectedness of flora and fauna at our campus. Explore information about the wildlife that depends on our local plant communities.

 Data under preparation!

Table: 1 Flora Database

SN	Flora	Em(IM)blem	MapLocat(ions)	Address(s)	Classification	InfoLite	Cataloging	InfoBase Status
1	Neem		https://maps.app.goo.gl/2keafQtLZ2ujptF18	Right front to Girls’ hostel	https://krishiprabha.blogspot.com/2024/03/neem-taxonomy.html	https://krishiprabha.blogspot.com/2024/03/neem-infobase.html	NO	YES
			https://maps.app.goo.gl/WGeiBaNb76MVgDaP8	Left front to Boys hostel
			https://maps.app.goo.gl/XpNZjgr6aX4FepYY8	Front to college canteen
2	Banana		https://maps.app.goo.gl/UiK3W6BL7c7FYuq56	Front to Girls’ hostel	https://krishiprabha.blogspot.com/2024/03/banana-taxonomy.html	https://krishiprabha.blogspot.com/2024/03/banana-infobase.html	YES	YES
			https://maps.app.goo.gl/GUjnZGRLdH3H3FQP6	Left to college building wall
			https://maps.app.goo.gl/BuGNBGDhhGyBa1dw6	Left front of college building
			https://maps.app.goo.gl/BsFE5i87AuYHQ6hM9	Front gate of college building
3	Royal palm(s)		https://maps.app.goo.gl/di54inoY2LzN2GhJ8	Front gate of college building	https://krishiprabha.blogspot.com/2024/03/royal-palm-roystonea-regia-taxonomy.html	https://krishiprabha.blogspot.com/2024/03/royal-palm-roystonea-regia-infobase.html	YES	YES
4	Badam tree		https://maps.app.goo.gl/qfiGhwd1uP7ZPGgVA	Right front to college building	https://krishiprabha.blogspot.com/2024/03/badam-tree-prunus-dulcis-taxonomy.html	https://krishiprabha.blogspot.com/2024/03/badam-tree-prunus-dulcis-infobase.html	NO	YES
5	Lemon tree		Currently Not Available	Currently Not Available	https://krishiprabha.blogspot.com/2024/03/lemon-tree-citrus-limon-taxonomy.html	https://krishiprabha.blogspot.com/2024/03/lemon-tree-citrus-limon-taxonomy_11.html	-	YES
6	Peepal		-	-	-	-	-	-
7	Teak tree		-	-	-	-	-	-
8	Gulmohor		-	-	-	-	-	-
9	Rose		-	-	-	-	-	-
10	Custard apple		-	-	-	-	-	-
11	Tamarind		-	-	-	-	-	-

 

Reference: